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This raised the question of whether vesicles normally targeted to the tonoplast are mistargeted in infected cells or, alternatively, the specificity of their fusion is lost. 7 B, lane 2). 2 B, bottom panel, lanes 2 and 4). They found that cotransfection of HeLa cells with an N-terminally truncated Chmp2a with the ATPase-negative Skd1 mutant inhibited formation of the aberrant membrane compartment, suggesting that the N-terminal coiled-coil portion of Chmp2a directed membrane association of the mutant Skd1 protein. In sharp contrast, interaction of PalB with DidBDid2 was barely above detection ( Fig. Yahoo free web page hosting only can I test yahoo free web page hosting PHP scripts on your server, I can also edit my files in your text editor, save it in here and then run them on the subdomain. 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